Coding

Part:BBa_K1152009:Design

Designed by: Ralf Beer, Konrad Herbst, Nikolaos Ignatiadis   Group: iGEM13_Heidelberg   (2013-09-19)

Sfp 4'-Phosphopanthetheinyl-transferase

  • CDS of sfp derived from Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 651
    Illegal SapI.rc site found at 661


Design Notes

The sfp CDS is derived from a surfactant producing derivative of B. subtilis wt JH642. [Nakano, 1992] We cloned sfp in a pSB3K3-derived plasmid with a lac promoter (BBa_R0010) and the RBS BBa_B0029.

Source

Sfp originally comes from genome of Bacillus subtilis strain OKB170. We amplified it from E. coli BAP1 which carries the sfp gene.

References

  • Lambalot RH, Gehring AM, Flugel RS, Zuber P, LaCelle M, Marahiel MA, Reid R, Khosla C, Walsh CT (1996) A new enzyme superfamily - the phosphopantetheinyl transferases. Chem Biol 3: 923-936.
  • Nakano, M. M., Corbell, N., Besson, J., & Zuber, P. (1992). Isolation and characterization of sfp: a gene that functions in the production of the lipopeptide biosurfactant, surfactin, in Bacillus subtilis. Molecular & general genetics : MGG, 232(2), 313–21. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/1557038
  • Pfeifer, B. a, Admiraal, S. J., Gramajo, H., Cane, D. E., & Khosla, C. (2001). Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli. Science (New York, N.Y.), 291(5509), 1790–2. doi:10.1126/science.1058092